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phycoerythrin labelled antibodies against cxcr4  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology phycoerythrin labelled antibodies against cxcr4
    Genome-editing activity of SpCas9 and SpRYc in CEM-R5 T cells. ( A ) The localization of the tested target sites at the C–X–C motif chemokine receptor 4 ( <t>CXCR4</t> ) and beta-2-microglobulin ( B2M ) loci. ( B ) CXCR4 KO efficiency measured with different PAMs. ( C ) B2M KO efficiency measured with different PAMs. To compare each experimental group at CXCR4 and B2M loci, a one-way ANOVA with Dunnett’s multiple comparison test was used. Data are presented as the mean ± SD. Significance: ns, not significant; **, p < 0.01; ****, p < 0.0001.
    Phycoerythrin Labelled Antibodies Against Cxcr4, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phycoerythrin+labelled+antibodies+against+cxcr4/PE+Anti-Human+CD184%2FCXCR4+Antibody/pmc12787127-208-12-17
    Average 94 stars, based on 6 article reviews
    phycoerythrin labelled antibodies against cxcr4 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems"

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms27010488

    Genome-editing activity of SpCas9 and SpRYc in CEM-R5 T cells. ( A ) The localization of the tested target sites at the C–X–C motif chemokine receptor 4 ( CXCR4 ) and beta-2-microglobulin ( B2M ) loci. ( B ) CXCR4 KO efficiency measured with different PAMs. ( C ) B2M KO efficiency measured with different PAMs. To compare each experimental group at CXCR4 and B2M loci, a one-way ANOVA with Dunnett’s multiple comparison test was used. Data are presented as the mean ± SD. Significance: ns, not significant; **, p < 0.01; ****, p < 0.0001.
    Figure Legend Snippet: Genome-editing activity of SpCas9 and SpRYc in CEM-R5 T cells. ( A ) The localization of the tested target sites at the C–X–C motif chemokine receptor 4 ( CXCR4 ) and beta-2-microglobulin ( B2M ) loci. ( B ) CXCR4 KO efficiency measured with different PAMs. ( C ) B2M KO efficiency measured with different PAMs. To compare each experimental group at CXCR4 and B2M loci, a one-way ANOVA with Dunnett’s multiple comparison test was used. Data are presented as the mean ± SD. Significance: ns, not significant; **, p < 0.01; ****, p < 0.0001.

    Techniques Used: Activity Assay, Comparison

    Related Articles

    Immunofluorescence:

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems
    Article Snippet: .. For immunofluorescence staining, cells were washed once with PBS and incubated with phycoerythrin-labelled antibodies against CXCR4 (#E-AB-F1157D, Elabscience, Houston, TX, USA) or B2M (#316306, Biolegend, San Diego, CA, USA) diluted in PBS at 4 °C for 30 min. Then, cells were washed twice with PBS and analyzed using a CytoFLEX S flow cytometer (Beckman-Coulter, USA). .. Data were acquired and analyzed by CytExpert 2.0 software (Beckman-Coulter, USA).

    Staining:

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems
    Article Snippet: .. For immunofluorescence staining, cells were washed once with PBS and incubated with phycoerythrin-labelled antibodies against CXCR4 (#E-AB-F1157D, Elabscience, Houston, TX, USA) or B2M (#316306, Biolegend, San Diego, CA, USA) diluted in PBS at 4 °C for 30 min. Then, cells were washed twice with PBS and analyzed using a CytoFLEX S flow cytometer (Beckman-Coulter, USA). .. Data were acquired and analyzed by CytExpert 2.0 software (Beckman-Coulter, USA).

    Incubation:

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems
    Article Snippet: .. For immunofluorescence staining, cells were washed once with PBS and incubated with phycoerythrin-labelled antibodies against CXCR4 (#E-AB-F1157D, Elabscience, Houston, TX, USA) or B2M (#316306, Biolegend, San Diego, CA, USA) diluted in PBS at 4 °C for 30 min. Then, cells were washed twice with PBS and analyzed using a CytoFLEX S flow cytometer (Beckman-Coulter, USA). .. Data were acquired and analyzed by CytExpert 2.0 software (Beckman-Coulter, USA).

    Flow Cytometry:

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems
    Article Snippet: .. For immunofluorescence staining, cells were washed once with PBS and incubated with phycoerythrin-labelled antibodies against CXCR4 (#E-AB-F1157D, Elabscience, Houston, TX, USA) or B2M (#316306, Biolegend, San Diego, CA, USA) diluted in PBS at 4 °C for 30 min. Then, cells were washed twice with PBS and analyzed using a CytoFLEX S flow cytometer (Beckman-Coulter, USA). .. Data were acquired and analyzed by CytExpert 2.0 software (Beckman-Coulter, USA).



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    Genome-editing activity of SpCas9 and SpRYc in CEM-R5 T cells. ( A ) The localization of the tested target sites at the C–X–C motif chemokine receptor 4 ( <t>CXCR4</t> ) and beta-2-microglobulin ( B2M ) loci. ( B ) CXCR4 KO efficiency measured with different PAMs. ( C ) B2M KO efficiency measured with different PAMs. To compare each experimental group at CXCR4 and B2M loci, a one-way ANOVA with Dunnett’s multiple comparison test was used. Data are presented as the mean ± SD. Significance: ns, not significant; **, p < 0.01; ****, p < 0.0001.
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    Image Search Results


    Genome-editing activity of SpCas9 and SpRYc in CEM-R5 T cells. ( A ) The localization of the tested target sites at the C–X–C motif chemokine receptor 4 ( CXCR4 ) and beta-2-microglobulin ( B2M ) loci. ( B ) CXCR4 KO efficiency measured with different PAMs. ( C ) B2M KO efficiency measured with different PAMs. To compare each experimental group at CXCR4 and B2M loci, a one-way ANOVA with Dunnett’s multiple comparison test was used. Data are presented as the mean ± SD. Significance: ns, not significant; **, p < 0.01; ****, p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: The Chimeric Nuclease SpRYc Exhibits Highly Variable Performance Across Biological Systems

    doi: 10.3390/ijms27010488

    Figure Lengend Snippet: Genome-editing activity of SpCas9 and SpRYc in CEM-R5 T cells. ( A ) The localization of the tested target sites at the C–X–C motif chemokine receptor 4 ( CXCR4 ) and beta-2-microglobulin ( B2M ) loci. ( B ) CXCR4 KO efficiency measured with different PAMs. ( C ) B2M KO efficiency measured with different PAMs. To compare each experimental group at CXCR4 and B2M loci, a one-way ANOVA with Dunnett’s multiple comparison test was used. Data are presented as the mean ± SD. Significance: ns, not significant; **, p < 0.01; ****, p < 0.0001.

    Article Snippet: For immunofluorescence staining, cells were washed once with PBS and incubated with phycoerythrin-labelled antibodies against CXCR4 (#E-AB-F1157D, Elabscience, Houston, TX, USA) or B2M (#316306, Biolegend, San Diego, CA, USA) diluted in PBS at 4 °C for 30 min. Then, cells were washed twice with PBS and analyzed using a CytoFLEX S flow cytometer (Beckman-Coulter, USA).

    Techniques: Activity Assay, Comparison